The player does not control the duplicants directly, and instead provides prioritized instructions, from which the duplicants will then follow to the best of their abilities. To help establish the colony, the player directs the duplicants to perform certain tasks, such as mining for resources, growing food, crafting equipment, researching new technologies, and maintaining their own health through nourishment, rest, and hygiene. The game simulates the diffusion of gases and equalization of atmospheres when a new natural chamber is opened, which can cause oxygen levels to drop in existing chambers, as well as the draining of liquids by gravity. While initial areas include a breathable atmosphere, areas within the world are in a vacuum or lack oxygen, requiring proper preparation by the duplicants before they explore into these areas. Each game's world is procedurally generated. The player must monitor the duplicants' hunger, waste, and oxygen levels to keep them alive. The player is tasked with managing and taking care of these duplicants as they try to survive and create a sustainable makeshift space colony. At the start of a new game, three duplicants find themselves in an asteroid with isolated pockets of breathable atmosphere, with no memory how they got there.

Oxygen Not Included is a simulation survival game. After being released on Steam's early access since February 2017, the final version of the game was released on July 30, 2019. Oxygen Not Included is a survival simulation video game developed and published by Klei Entertainment.

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